Research Profiles: SECM meets fluorescence microscopy
نویسندگان
چکیده
منابع مشابه
Fluorescence and fluorescence microscopy
The concept of specific cellular antigen staining by use of an immunofluorescent technique was introduced by AH COONS and co-workers about 60 years ago. Fluorescent probes are efficient tools and enable the detection of particular components in complex structures of organs including live cells. Under the condition of specific molecular interactions, fluorochrome labeled ligands allow the select...
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Scanning electrochemical microscopy (SECM) experiments were undertaken to study the EC’mechanism, e.g. where a product of the electrode reaction reacts to regenerate the starting material. Digital simulations of the two dimensional SECM were carried out and the results tested with the known EC’ system of Fe(CN)6 / cysteine for validation. The results were then used in SECM experiments with Ru(p...
متن کاملFluorescence Microscopy
When organic or inorganic specimens absorb and subsequently reradiate light, the process is typically a result of fluorescence or phosphorescence. Fluorescence emission is nearly simultaneous with the absorption of the excitation light as the time delay between photon absorption and emission is typically less than a microsecond. When the emission persists long after the excitation light is exti...
متن کاملFluorescence Microscopy
The human eye requires contrast to perceive details of objects. Several ingenious methods of improving the contrast of microscopy images have been designed, and each of them opened new applications of optical microscopy in biology. The simplest and very effective contrasting method is ‘‘dark field.’’ It exploits the scattering of light on small particles that differ from their environment in re...
متن کاملConfocal scanning fluorescence microscopy: a new method for phagocytosis research.
An important new method for phagocytosis research, confocal scanning fluorescence light microscopy (CSFM), is demonstrated using fluorescent microspheres ingested by murine macrophages. CSFM, in combination with Nomarski differential interference contrast microscopy (DIC), can resolve microspheres inside cells from microspheres attached to the surface of cells. Further, combined CSFM and DIC im...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2004
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac041588l